Calculate how much of this gravel is required to attain a specific depth in a cylindrical, quarter cylindrical or in a rectangular shaped aquarium or pond weight to volume volume to weight price Metaphosphoric acid HPO 3 weighs 2 200 kgm (137.34151 lbft) weight to volume volume to weight price mole to volume and weight density Volume to weight, weight to volume and cost conversions for Refrigerant R-11, liquid (R11) with temperature in the range of -1.12C (29.984F) to 115.56C (240.008F) Weights and Measurements A tonne also referred to as a metric tonne, is a unit of mass equal to 1000 kg, or approximately the mass of one cubic meter of water at four degrees Celsius The radiation absorbed dose is a measurement of radiation, in energy per unit of mass, absorbed by a specific object, such as human tissue.Calculators The present value of an annuity and annuity payments calculator home Gt; conversion tables of units of measurements Gt; conversion tables of units of weight Gt; conversion tables of micrograms Gt; conversion table of micrograms to kilograms Privacy Policy Terms and Conditions News Contact Us About Us 2020 AVCalc LLC.All rights reserved worldwide.
Cite 5 Recommendations 13th Jan, 2016 Biruk Tesfaye Birhanu Kyungpook National University I think there is some difference between directly counting the glycerol stock and the activated bacteria (after 2 or 3 times culture in broth). I will test an antibacterial surface so I have to know how many bacteria there are in the LB medium before putting them onto the surface. Is there a way to calculate it Antibacterials Glycerol Bacteria Share Facebook Twitter LinkedIn Reddit Most recent answer 25th Aug, 2020 Ban M. S. Saeed Al-Zahraa Medical College University of Basrah To find out the number of CFU ml in the original sample, the number of colony forming units on the countable plate is multiplied by 1FDF. This takes into account all of the dilution of the original sample.. ![]() Cite Popular Answers (1) 9th Jan, 2016 Rafik Karaman Al-Quds University Dear Ozge, For calculating the colony forming unit bacteria, please see the following text: colony-forming unit (CFU or cfu) is a measure of viable bacterial or fungal cells. In direct microscopic counts (cell counting using haemocytometer) where all cells, dead and living, are counted,but CFU measures only viable cells. For convenience the results are given as CFUmL (colony-forming units per milliliter) for liquids, and CFUg (colony-forming units per gram) for solids. CFU can be calculated using miles and misra method, it is useful to determine the microbiological load and magnitude of infection in blood and other samples. Example: Calculate the number of bacteria (CFU) per milliliter or gram of sample by dividing the number of colonies by the dilution factor The number of colonies per ml reported should reflect the precision of the method and should not include more than two significant figures. Then, the number of bacteria in 1 ml of the original sample can be calculated as follows: Bacteriaml (130) x (106) 1.3 108 or 130,000,000. ![]() From this suspension, two serial, 1100 dilutions are made, and 0.1 ml is plated onto Plate Count Agar from the last dilution. Calculate CFUmL of the original Sample Answer: First thing we need to know is the Dilution Factor, or how much the original sample is diluted: here Initially 5mL in 45mL Final Volume Sample volume 505 10. For illustration of the figures in this article, please use the following link: Hoping this will be helpful, Rafik Cite 151 Recommendations All Answers (80) 9th Jan, 2016 Rafik Karaman Al-Quds University Dear Ozge, For calculating the colony forming unit bacteria, please see the following text: colony-forming unit (CFU or cfu) is a measure of viable bacterial or fungal cells. For illustration of the figures in this article, please use the following link: Hoping this will be helpful, Rafik Cite 151 Recommendations Deleted profile I hope Rafiks reply might have solved your problem, you need not to opt for any specific method for glycerol stocks it is same as for the other broths. You may read more in: Labtop for Microbiology Laboratory at the link provided. Book Labtop for Microbiology Laboratory Cite 2 Recommendations 10th Jan, 2016 Adam B Shapiro Entasis therapeutics Basically, you have to measure it by plating out the various dilutions of the glycerol stock on agar plates made in growth medium and counting the colonies.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |